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Question category: Haematology

A 9 year old girl is brought to her GP. Her mother has noticed that she appears jaundice. She is feeling very tired. This has happened intermittently on several occasions. On examination she appears jaundice and there is evidence of splenomegaly. Bloods reveal an elevated unconjugated bilirubin and LDH. There is evidence of an elevated reticulocyte count. A blood film reveals reticulocytes and spherocytes. Which of the following is the most useful in differentiating hereditary spherocytosis from autoimmune haemolytic anaemia?


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